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Sangon Biotech pucm t vectors
Pucm T Vectors, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pucm-t+vector/pucm+t+vectors/bio_rxiv__2025__11__17__688744-32-5-7
Average 86 stars, based on 1 article reviews
pucm t vectors - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Occurrence, Distribution, and Genetic Diversity of Alfalfa ( Medicago sativa L.) Viruses in Four Major Alfalfa-Producing Provinces of China
Article Snippet: The purified PCR products consisting of the sequence fragments of virus capsid protein were cloned into the pUCm-T vector (Sangon Biotech) and transformed into Escherichia coli DH5α competent cells (Sangon Biotech).

Article Title: Association between metabolic profile and microbiomic changes in rats with functional dyspepsia
Article Snippet: The purified PCR products were cloned into Escherichia coli DH5α using pUCm-T Vector (Sangon Biotech, Shanghai, China).

Article Title: Differential DNA methylation landscape of miRNAs genes in mice liver fibrosis
Article Snippet: Ampli ed DNA fragments were ligated into pUCm-T vector (Sangon Biotech, China), and then the T-vector was transformed into competent JM109 E. coli cells.

Article Title: A Global Analysis of Y-STR INRA189 Polymorphism in Chinese Domestic Yak Breeds/Populations
Article Snippet: To confirm the sequence characterization of each allele by DNA sequencing, we cloned PCR amplicons from different alleles into the pUCm-T vector (Sangon Biotech Co., Ltd., Shanghai, China) and carried out sequence alignment using BioEdit v7.2.5 software with Clustal W multiple alignment [ ].

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin.
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: A metabolic labeling method detects m 6 A transcriptome-wide at single base resolution.
Article Snippet: 1MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.. 2College of Animal Sciences, Key Laboratory of Animal Nutrition & Feed Sciences, Ministry of Agriculture, Zhejiang University, Hangzhou, China.. 3Institute of Biology, Westlake Institute for Advanced Study, School of Life Sciences, Westlake University, Hangzhou, Zhejiang Province, China.

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: Diversity and community structure of ammonia oxidizers in a marsh wetland of the northeast China.
Article Snippet: As an interface of terrestrial and aquatic ecosystems, wetland is a hotspot of the global nitrogen cycle.. Ammonia oxidation is an essential part of the nitrogen cycle and is conducted by ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB).. Based on the amoA gene, the distribution and genetic diversity of AOA and AOB in the marsh wetland soil with different soil layers and vegetation had been investigated.

Plasmid Preparation:

Article Title: Occurrence, Distribution, and Genetic Diversity of Alfalfa ( Medicago sativa L.) Viruses in Four Major Alfalfa-Producing Provinces of China
Article Snippet: The purified PCR products consisting of the sequence fragments of virus capsid protein were cloned into the pUCm-T vector (Sangon Biotech) and transformed into Escherichia coli DH5α competent cells (Sangon Biotech).

Article Title: Association between metabolic profile and microbiomic changes in rats with functional dyspepsia
Article Snippet: The purified PCR products were cloned into Escherichia coli DH5α using pUCm-T Vector (Sangon Biotech, Shanghai, China).

Article Title: Differential DNA methylation landscape of miRNAs genes in mice liver fibrosis
Article Snippet: Ampli ed DNA fragments were ligated into pUCm-T vector (Sangon Biotech, China), and then the T-vector was transformed into competent JM109 E. coli cells.

Article Title: A Global Analysis of Y-STR INRA189 Polymorphism in Chinese Domestic Yak Breeds/Populations
Article Snippet: To confirm the sequence characterization of each allele by DNA sequencing, we cloned PCR amplicons from different alleles into the pUCm-T vector (Sangon Biotech Co., Ltd., Shanghai, China) and carried out sequence alignment using BioEdit v7.2.5 software with Clustal W multiple alignment [ ].

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin.
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: A metabolic labeling method detects m 6 A transcriptome-wide at single base resolution.
Article Snippet: 1MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.. 2College of Animal Sciences, Key Laboratory of Animal Nutrition & Feed Sciences, Ministry of Agriculture, Zhejiang University, Hangzhou, China.. 3Institute of Biology, Westlake Institute for Advanced Study, School of Life Sciences, Westlake University, Hangzhou, Zhejiang Province, China.

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: Diversity and community structure of ammonia oxidizers in a marsh wetland of the northeast China.
Article Snippet: As an interface of terrestrial and aquatic ecosystems, wetland is a hotspot of the global nitrogen cycle.. Ammonia oxidation is an essential part of the nitrogen cycle and is conducted by ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB).. Based on the amoA gene, the distribution and genetic diversity of AOA and AOB in the marsh wetland soil with different soil layers and vegetation had been investigated.

Transformation Assay:

Article Title: Occurrence, Distribution, and Genetic Diversity of Alfalfa ( Medicago sativa L.) Viruses in Four Major Alfalfa-Producing Provinces of China
Article Snippet: The purified PCR products consisting of the sequence fragments of virus capsid protein were cloned into the pUCm-T vector (Sangon Biotech) and transformed into Escherichia coli DH5α competent cells (Sangon Biotech).

Article Title: Association between metabolic profile and microbiomic changes in rats with functional dyspepsia
Article Snippet: The purified PCR products were cloned into Escherichia coli DH5α using pUCm-T Vector (Sangon Biotech, Shanghai, China).

Article Title: Differential DNA methylation landscape of miRNAs genes in mice liver fibrosis
Article Snippet: Ampli ed DNA fragments were ligated into pUCm-T vector (Sangon Biotech, China), and then the T-vector was transformed into competent JM109 E. coli cells.

Article Title: A Global Analysis of Y-STR INRA189 Polymorphism in Chinese Domestic Yak Breeds/Populations
Article Snippet: To confirm the sequence characterization of each allele by DNA sequencing, we cloned PCR amplicons from different alleles into the pUCm-T vector (Sangon Biotech Co., Ltd., Shanghai, China) and carried out sequence alignment using BioEdit v7.2.5 software with Clustal W multiple alignment [ ].

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin.
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: A metabolic labeling method detects m 6 A transcriptome-wide at single base resolution.
Article Snippet: 1MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.. 2College of Animal Sciences, Key Laboratory of Animal Nutrition & Feed Sciences, Ministry of Agriculture, Zhejiang University, Hangzhou, China.. 3Institute of Biology, Westlake Institute for Advanced Study, School of Life Sciences, Westlake University, Hangzhou, Zhejiang Province, China.

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: Diversity and community structure of ammonia oxidizers in a marsh wetland of the northeast China.
Article Snippet: As an interface of terrestrial and aquatic ecosystems, wetland is a hotspot of the global nitrogen cycle.. Ammonia oxidation is an essential part of the nitrogen cycle and is conducted by ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB).. Based on the amoA gene, the distribution and genetic diversity of AOA and AOB in the marsh wetland soil with different soil layers and vegetation had been investigated.

Purification:

Article Title: Occurrence, Distribution, and Genetic Diversity of Alfalfa ( Medicago sativa L.) Viruses in Four Major Alfalfa-Producing Provinces of China
Article Snippet: The purified PCR products consisting of the sequence fragments of virus capsid protein were cloned into the pUCm-T vector (Sangon Biotech) and transformed into Escherichia coli DH5α competent cells (Sangon Biotech).

Article Title: Association between metabolic profile and microbiomic changes in rats with functional dyspepsia
Article Snippet: The purified PCR products were cloned into Escherichia coli DH5α using pUCm-T Vector (Sangon Biotech, Shanghai, China).

Article Title: Differential DNA methylation landscape of miRNAs genes in mice liver fibrosis
Article Snippet: Ampli ed DNA fragments were ligated into pUCm-T vector (Sangon Biotech, China), and then the T-vector was transformed into competent JM109 E. coli cells.

Article Title: A Global Analysis of Y-STR INRA189 Polymorphism in Chinese Domestic Yak Breeds/Populations
Article Snippet: To confirm the sequence characterization of each allele by DNA sequencing, we cloned PCR amplicons from different alleles into the pUCm-T vector (Sangon Biotech Co., Ltd., Shanghai, China) and carried out sequence alignment using BioEdit v7.2.5 software with Clustal W multiple alignment [ ].

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin.
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: A metabolic labeling method detects m 6 A transcriptome-wide at single base resolution.
Article Snippet: 1MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.. 2College of Animal Sciences, Key Laboratory of Animal Nutrition & Feed Sciences, Ministry of Agriculture, Zhejiang University, Hangzhou, China.. 3Institute of Biology, Westlake Institute for Advanced Study, School of Life Sciences, Westlake University, Hangzhou, Zhejiang Province, China.

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: Diversity and community structure of ammonia oxidizers in a marsh wetland of the northeast China.
Article Snippet: As an interface of terrestrial and aquatic ecosystems, wetland is a hotspot of the global nitrogen cycle.. Ammonia oxidation is an essential part of the nitrogen cycle and is conducted by ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB).. Based on the amoA gene, the distribution and genetic diversity of AOA and AOB in the marsh wetland soil with different soil layers and vegetation had been investigated.

Sequencing:

Article Title: Occurrence, Distribution, and Genetic Diversity of Alfalfa ( Medicago sativa L.) Viruses in Four Major Alfalfa-Producing Provinces of China
Article Snippet: The purified PCR products consisting of the sequence fragments of virus capsid protein were cloned into the pUCm-T vector (Sangon Biotech) and transformed into Escherichia coli DH5α competent cells (Sangon Biotech).

Article Title: Association between metabolic profile and microbiomic changes in rats with functional dyspepsia
Article Snippet: The purified PCR products were cloned into Escherichia coli DH5α using pUCm-T Vector (Sangon Biotech, Shanghai, China).

Article Title: Differential DNA methylation landscape of miRNAs genes in mice liver fibrosis
Article Snippet: Ampli ed DNA fragments were ligated into pUCm-T vector (Sangon Biotech, China), and then the T-vector was transformed into competent JM109 E. coli cells.

Article Title: A Global Analysis of Y-STR INRA189 Polymorphism in Chinese Domestic Yak Breeds/Populations
Article Snippet: To confirm the sequence characterization of each allele by DNA sequencing, we cloned PCR amplicons from different alleles into the pUCm-T vector (Sangon Biotech Co., Ltd., Shanghai, China) and carried out sequence alignment using BioEdit v7.2.5 software with Clustal W multiple alignment [ ].

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin.
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: A metabolic labeling method detects m 6 A transcriptome-wide at single base resolution.
Article Snippet: 1MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.. 2College of Animal Sciences, Key Laboratory of Animal Nutrition & Feed Sciences, Ministry of Agriculture, Zhejiang University, Hangzhou, China.. 3Institute of Biology, Westlake Institute for Advanced Study, School of Life Sciences, Westlake University, Hangzhou, Zhejiang Province, China.

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: Diversity and community structure of ammonia oxidizers in a marsh wetland of the northeast China.
Article Snippet: As an interface of terrestrial and aquatic ecosystems, wetland is a hotspot of the global nitrogen cycle.. Ammonia oxidation is an essential part of the nitrogen cycle and is conducted by ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB).. Based on the amoA gene, the distribution and genetic diversity of AOA and AOB in the marsh wetland soil with different soil layers and vegetation had been investigated.

Clone Assay:

Article Title: Occurrence, Distribution, and Genetic Diversity of Alfalfa ( Medicago sativa L.) Viruses in Four Major Alfalfa-Producing Provinces of China
Article Snippet: The purified PCR products consisting of the sequence fragments of virus capsid protein were cloned into the pUCm-T vector (Sangon Biotech) and transformed into Escherichia coli DH5α competent cells (Sangon Biotech).

Article Title: Association between metabolic profile and microbiomic changes in rats with functional dyspepsia
Article Snippet: The purified PCR products were cloned into Escherichia coli DH5α using pUCm-T Vector (Sangon Biotech, Shanghai, China).

Article Title: Differential DNA methylation landscape of miRNAs genes in mice liver fibrosis
Article Snippet: Ampli ed DNA fragments were ligated into pUCm-T vector (Sangon Biotech, China), and then the T-vector was transformed into competent JM109 E. coli cells.

Article Title: A Global Analysis of Y-STR INRA189 Polymorphism in Chinese Domestic Yak Breeds/Populations
Article Snippet: To confirm the sequence characterization of each allele by DNA sequencing, we cloned PCR amplicons from different alleles into the pUCm-T vector (Sangon Biotech Co., Ltd., Shanghai, China) and carried out sequence alignment using BioEdit v7.2.5 software with Clustal W multiple alignment [ ].

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin.
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: A metabolic labeling method detects m 6 A transcriptome-wide at single base resolution.
Article Snippet: 1MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.. 2College of Animal Sciences, Key Laboratory of Animal Nutrition & Feed Sciences, Ministry of Agriculture, Zhejiang University, Hangzhou, China.. 3Institute of Biology, Westlake Institute for Advanced Study, School of Life Sciences, Westlake University, Hangzhou, Zhejiang Province, China.

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: Diversity and community structure of ammonia oxidizers in a marsh wetland of the northeast China.
Article Snippet: As an interface of terrestrial and aquatic ecosystems, wetland is a hotspot of the global nitrogen cycle.. Ammonia oxidation is an essential part of the nitrogen cycle and is conducted by ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB).. Based on the amoA gene, the distribution and genetic diversity of AOA and AOB in the marsh wetland soil with different soil layers and vegetation had been investigated.

DNA Sequencing:

Article Title: Occurrence, Distribution, and Genetic Diversity of Alfalfa ( Medicago sativa L.) Viruses in Four Major Alfalfa-Producing Provinces of China
Article Snippet: The purified PCR products consisting of the sequence fragments of virus capsid protein were cloned into the pUCm-T vector (Sangon Biotech) and transformed into Escherichia coli DH5α competent cells (Sangon Biotech).

Article Title: Association between metabolic profile and microbiomic changes in rats with functional dyspepsia
Article Snippet: The purified PCR products were cloned into Escherichia coli DH5α using pUCm-T Vector (Sangon Biotech, Shanghai, China).

Article Title: Differential DNA methylation landscape of miRNAs genes in mice liver fibrosis
Article Snippet: Ampli ed DNA fragments were ligated into pUCm-T vector (Sangon Biotech, China), and then the T-vector was transformed into competent JM109 E. coli cells.

Article Title: A Global Analysis of Y-STR INRA189 Polymorphism in Chinese Domestic Yak Breeds/Populations
Article Snippet: To confirm the sequence characterization of each allele by DNA sequencing, we cloned PCR amplicons from different alleles into the pUCm-T vector (Sangon Biotech Co., Ltd., Shanghai, China) and carried out sequence alignment using BioEdit v7.2.5 software with Clustal W multiple alignment [ ].

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin.
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: A metabolic labeling method detects m 6 A transcriptome-wide at single base resolution.
Article Snippet: 1MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.. 2College of Animal Sciences, Key Laboratory of Animal Nutrition & Feed Sciences, Ministry of Agriculture, Zhejiang University, Hangzhou, China.. 3Institute of Biology, Westlake Institute for Advanced Study, School of Life Sciences, Westlake University, Hangzhou, Zhejiang Province, China.

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: Diversity and community structure of ammonia oxidizers in a marsh wetland of the northeast China.
Article Snippet: As an interface of terrestrial and aquatic ecosystems, wetland is a hotspot of the global nitrogen cycle.. Ammonia oxidation is an essential part of the nitrogen cycle and is conducted by ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB).. Based on the amoA gene, the distribution and genetic diversity of AOA and AOB in the marsh wetland soil with different soil layers and vegetation had been investigated.

Software:

Article Title: Occurrence, Distribution, and Genetic Diversity of Alfalfa ( Medicago sativa L.) Viruses in Four Major Alfalfa-Producing Provinces of China
Article Snippet: The purified PCR products consisting of the sequence fragments of virus capsid protein were cloned into the pUCm-T vector (Sangon Biotech) and transformed into Escherichia coli DH5α competent cells (Sangon Biotech).

Article Title: Association between metabolic profile and microbiomic changes in rats with functional dyspepsia
Article Snippet: The purified PCR products were cloned into Escherichia coli DH5α using pUCm-T Vector (Sangon Biotech, Shanghai, China).

Article Title: Differential DNA methylation landscape of miRNAs genes in mice liver fibrosis
Article Snippet: Ampli ed DNA fragments were ligated into pUCm-T vector (Sangon Biotech, China), and then the T-vector was transformed into competent JM109 E. coli cells.

Article Title: A Global Analysis of Y-STR INRA189 Polymorphism in Chinese Domestic Yak Breeds/Populations
Article Snippet: To confirm the sequence characterization of each allele by DNA sequencing, we cloned PCR amplicons from different alleles into the pUCm-T vector (Sangon Biotech Co., Ltd., Shanghai, China) and carried out sequence alignment using BioEdit v7.2.5 software with Clustal W multiple alignment [ ].

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin.
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: A metabolic labeling method detects m 6 A transcriptome-wide at single base resolution.
Article Snippet: 1MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.. 2College of Animal Sciences, Key Laboratory of Animal Nutrition & Feed Sciences, Ministry of Agriculture, Zhejiang University, Hangzhou, China.. 3Institute of Biology, Westlake Institute for Advanced Study, School of Life Sciences, Westlake University, Hangzhou, Zhejiang Province, China.

Article Title: Identification, Characterization, Cloning, and Cross-Reactivity of Zan b 2, a Novel Pepper Allergen of 11S Legumin
Article Snippet: PCR products with dA-tailing were directly ligated into a pUCm-T vector (Sangon Biotech).

Article Title: Diversity and community structure of ammonia oxidizers in a marsh wetland of the northeast China.
Article Snippet: As an interface of terrestrial and aquatic ecosystems, wetland is a hotspot of the global nitrogen cycle.. Ammonia oxidation is an essential part of the nitrogen cycle and is conducted by ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB).. Based on the amoA gene, the distribution and genetic diversity of AOA and AOB in the marsh wetland soil with different soil layers and vegetation had been investigated.



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